singlecell rna expression profiling of ace2 and tmprss2 in the human trophectoderm CORD-Papers-2021-10-25 (Version 1)

Title: Singlecell RNA expression profiling of ACE2 and TMPRSS2 in the human trophectoderm and placenta
Abstract: OBJECTIVES: The objective of this study was to examine the characteristics and distributions of possible SARSCoV2 target cells in the human TE and placenta. METHODS: Singlecell transcriptomic datasets of the early TE as well as the first and secondtrimester placentas have been reported(1,2). Here, we conducted the transcriptomic analysis of 4198 early TE cells, 1260 firsttrimester placental cells and 189 EVTs at 24week placentas (EVT_24W) by SMARTSeq2 method. Immunohistochemical staining of the human first, second and third trimester placentas was performed to confirm bioinformatic results. RESULTS: Via bioinformatic analysis, we identified the existence of ACE2 and TMPRSS2 expression in human TE as well as in the first and second trimester placentas. In human TE data, 54.4% of TE1 cells, 9.0% of CTBs, 3.2% of EVTs and 29.5% of STBs were ACE2 positive. As for TMPRSS2, 90.7% of TE1 cells, 31.5% of CTBs, 22.1% of EVTs and 70.8% of STBs were TMPRSS2 positive. Amongst the placental cells, 20.4% of CTBs, 44.1% of STBs, 3.4% of EVT_8W and 63% of EVT_24W were ACE2 positive. And 1.6% of CTBs, 26.5% of STBs, 1.9% of EVT_8W and 20.1% of EVT_24W were TMPRSS2 positive. Pathway analysis revealed associations to morphogenesis of branching structure, extracellular matrix interaction, oxygen binding and antioxidant activity in ACE2+TMPRSS2+ EVT_24W cells. The ACE2+TMPRSS2+ TE1 cells were correlated with an increased capacity of viral invasion, epithelial cell proliferation and cell adhesion. Based on immunohistochemical results, expression level of ACE2 and TMPRSS2 in first and second and thirdtrimester placentas was observed. CONCLUSIONS: Our study has demonstrated the presence of ACE2 and TMPRSS2 positive cells in the human TE and placentas at different stages of pregnancy, which indicates the possibility that the SARSCoV2 could spread via the placenta and cause intrauterine fetal infection. This article is protected by copyright. All rights reserved.
Published: 8/27/2020
Journal: Ultrasound Obstet Gynecol
DOI: 10.1002/uog.22186
Author Name: Cui, D
Author link:
Author Name: Liu, Y
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Author Name: Jiang, X
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Author Name: Ding, C
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Author Name: Poon, L C
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Author Name: Wang, H
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Author Name: Yang, H
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license: no-cc
license_url: [no creative commons license associated]
source_x: Medline; PMC
pubmed_id: 32851697
pmcid: PMC7461088
has_full_text: FALSE
G_ID: singlecell_rna_expression_profiling_of_ace2_and_tmprss2_in_the_human_trophectoderm
S2 ID: 221343941