identification and characterization of a novel linear epitope in the spike protein CORD-Papers-2021-10-25 (Version 1)

Title: Identification and characterization of a novel linear epitope in the spike protein of the porcine epidemic diarrhea virus.
Abstract: The main immunogenic protein of the porcine epidemic diarrhea virus (PEDV) is the spike protein (S protein), which plays an important role in receptor binding, membrane fusion, and viral invasion of the host. In this paper, the linear epitope of the 3F10 non-neutralizing monoclonal antibody that was previously prepared in our laboratory was identified. The expression of truncated forms of the S protein for 3F10 reaction studies proved that the epitope was located between amino acids (aa) 674 and 791. To further locate the core aa of the 3F10 epitope, 12 random peptide libraries were used, and the result showed that the key aa located at aa 685-688 of the S protein (LLAF) were recognized by 3F10. Homology analysis of the regions corresponding to 20 typical strains of different PEDV subtypes showed that the epitope is highly conserved. Identifying the epitope recognized by an antibody helps to improve our understanding of the structure and function of the antigen. Keywords: spike protein; linear epitope; homology analysis.
Published: 2019
Journal: Acta virologica
DOI: 10.4149/av_2019_111
DOI_URL: http://doi.org/10.4149/av_2019_111
Author Name: Gong, L
Author link: https://covid19-data.nist.gov/pid/rest/local/author/gong_l
Author Name: Gong, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/gong_y
Author Name: Lin, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/lin_y
Author Name: Qin, J
Author link: https://covid19-data.nist.gov/pid/rest/local/author/qin_j
Author Name: Liu, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/liu_y
Author Name: Qian, X
Author link: https://covid19-data.nist.gov/pid/rest/local/author/qian_x
Author Name: Xue, C
Author link: https://covid19-data.nist.gov/pid/rest/local/author/xue_c
Author Name: Cao, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/cao_y
license: unk
license_url: [unknown license]
source_x: Medline
source_x_url: https://www.medline.com/
pubmed_id: 30879317
pubmed_id_url: https://www.ncbi.nlm.nih.gov/pubmed/30879317
url: https://doi.org/10.4149/av_2019_111 https://www.ncbi.nlm.nih.gov/pubmed/30879317/
has_full_text: FALSE
G_ID: identification_and_characterization_of_a_novel_linear_epitope_in_the_spike_protein
S2 ID: 81981592