duplicate us1 genes of duck enteritis virus encode a non essential immediate early CORD-Papers-2021-10-25 (Version 1)

Title: Duplicate US1 Genes of Duck Enteritis Virus Encode a Non-essential Immediate Early Protein Localized to the Nucleus
Abstract: The duplicate US1 genes of duck enteritis virus (DEV) encode a protein with a conserved Herpes_IE68 domain, which was found to be closely related to the herpes virus immediate early regulatory protein family and is highly conserved among counterparts encoded by Herpes_IE68 genes Previous studies found the homologous proteins HSV-1 ICP22 and VZV ORF63/ORF70 to be critical for virus transcription and replication However, little is known about the DEV ICP22 protein In this paper, we describe the characteristics of this protein based on pharmacological experiments, real-time quantitative Polymerase Chain Reaction, Western blot, and immunofluorescence assays We also investigate the role of the protein in DEV replication via mutation of US1 As a result, we found that the DEV ICP22 protein is a non-essential immediate early protein predominantly located in the nucleus of infected DEF cells and that DEV replication is impaired by US1 deletion We also found that ICP22 contains a classical nuclear localization signal (NLS) at 305-312AA, and ICP22 cannot enter the nucleus by itself after mutating residue 309 Copyright 2020 Li, Wu, Wang, Ma, Jia, Chen, Zhu, Liu, Yang, Zhao, Zhang, Huang, Ou, Mao, Zhang, Liu, Yu, Pan, Tian, Rehman, Chen and Cheng
Published: 2020
Journal: Frontiers in Cellular and Infection Microbiology
Author Name: Li, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/li_y
Author Name: Wu, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/wu_y
Author Name: Wang, M
Author link: https://covid19-data.nist.gov/pid/rest/local/author/wang_m
Author Name: Ma, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/ma_y
Author Name: Jia, R
Author link: https://covid19-data.nist.gov/pid/rest/local/author/jia_r
Author Name: Chen, S
Author link: https://covid19-data.nist.gov/pid/rest/local/author/chen_s
Author Name: Zhu, D
Author link: https://covid19-data.nist.gov/pid/rest/local/author/zhu_d
Author Name: Liu, M
Author link: https://covid19-data.nist.gov/pid/rest/local/author/liu_m
Author Name: Yang, Q
Author link: https://covid19-data.nist.gov/pid/rest/local/author/yang_q
Author Name: Zhao, X
Author link: https://covid19-data.nist.gov/pid/rest/local/author/zhao_x
Author Name: Zhang, S
Author link: https://covid19-data.nist.gov/pid/rest/local/author/zhang_s
Author Name: Huang, J
Author link: https://covid19-data.nist.gov/pid/rest/local/author/huang_j
Author Name: Ou, X
Author link: https://covid19-data.nist.gov/pid/rest/local/author/ou_x
Author Name: Mao, S
Author link: https://covid19-data.nist.gov/pid/rest/local/author/mao_s
Author Name: Zhang, L
Author link: https://covid19-data.nist.gov/pid/rest/local/author/zhang_l
Author Name: Liu, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/liu_y
Author Name: Yu, Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/yu_y
Author Name: Pan, L
Author link: https://covid19-data.nist.gov/pid/rest/local/author/pan_l
Author Name: Tian, B
Author link: https://covid19-data.nist.gov/pid/rest/local/author/tian_b
Author Name: Rehman, M U
Author link: https://covid19-data.nist.gov/pid/rest/local/author/rehman_m_u
Author Name: Chen, X
Author link: https://covid19-data.nist.gov/pid/rest/local/author/chen_x
Author Name: Cheng, A
Author link: https://covid19-data.nist.gov/pid/rest/local/author/cheng_a
license: unk
license_url: [unknown license]
source_x: WHO
source_x_url: https://www.who.int/
who_covidence_id: #827748
has_full_text: FALSE
G_ID: duplicate_us1_genes_of_duck_enteritis_virus_encode_a_non_essential_immediate_early
S2 ID: 210693768