a non competing pair of human neutralizing antibodies block covid 19 virus binding CORD-Papers-2022-06-02 (Version 1)

Title: A non-competing pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Abstract: Neutralizing antibodies could be antivirals against COVID-19 pandemics. Here we report the isolation of four human-origin monoclonal antibodies from a convalescent patient in China. All of these isolated antibodies display neutralization abilities in vitro. Two of them (B38 and H4) block the binding between RBD and vial cellular receptor ACE2. Further competition assay indicates that B38 and H4 recognize different epitopes on the RBD which is ideal for a virus-targeting mAb-pair to avoid immune escape in the future clinical applications. Moreover therapeutic study on the mouse model validated that these two antibodies can reduce virus titers in the infected mouse lungs. Structure of RBD-B38 complex revealed that most residues on the epitope are overlapped with the RBD-ACE2 binding interface which explained the blocking efficacy and neutralizing capacity. Our results highlight the promise of antibody-based therapeutics and provide the structural basis of rational vaccine design.
Published: 2020-05-07
DOI: 10.1101/2020.05.01.20077743
DOI_URL: http://doi.org/10.1101/2020.05.01.20077743
Author Name: Wu Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/wu_y
Author Name: Wang F
Author link: https://covid19-data.nist.gov/pid/rest/local/author/wang_f
Author Name: Shen C
Author link: https://covid19-data.nist.gov/pid/rest/local/author/shen_c
Author Name: Peng W
Author link: https://covid19-data.nist.gov/pid/rest/local/author/peng_w
Author Name: Li D
Author link: https://covid19-data.nist.gov/pid/rest/local/author/li_d
Author Name: Zhao C
Author link: https://covid19-data.nist.gov/pid/rest/local/author/zhao_c
Author Name: Li Z
Author link: https://covid19-data.nist.gov/pid/rest/local/author/li_z
Author Name: Li S
Author link: https://covid19-data.nist.gov/pid/rest/local/author/li_s
Author Name: Bi Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/bi_y
Author Name: Yang Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/yang_y
Author Name: Gong Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/gong_y
Author Name: Xiao H
Author link: https://covid19-data.nist.gov/pid/rest/local/author/xiao_h
Author Name: Fan Z
Author link: https://covid19-data.nist.gov/pid/rest/local/author/fan_z
Author Name: Tan S
Author link: https://covid19-data.nist.gov/pid/rest/local/author/tan_s
Author Name: Wu G
Author link: https://covid19-data.nist.gov/pid/rest/local/author/wu_g
Author Name: Tan W
Author link: https://covid19-data.nist.gov/pid/rest/local/author/tan_w
Author Name: Lu X
Author link: https://covid19-data.nist.gov/pid/rest/local/author/lu_x
Author Name: Fan C
Author link: https://covid19-data.nist.gov/pid/rest/local/author/fan_c
Author Name: Wang Q
Author link: https://covid19-data.nist.gov/pid/rest/local/author/wang_q
Author Name: Liu Y
Author link: https://covid19-data.nist.gov/pid/rest/local/author/liu_y
Author Name: Qi J
Author link: https://covid19-data.nist.gov/pid/rest/local/author/qi_j
Author Name: Gao G F
Author link: https://covid19-data.nist.gov/pid/rest/local/author/gao_g_f
Author Name: Gao F
Author link: https://covid19-data.nist.gov/pid/rest/local/author/gao_f
Author Name: Liu L
Author link: https://covid19-data.nist.gov/pid/rest/local/author/liu_l
license: medrxiv
source_x: MedRxiv; WHO
source_x_url: https://www.who.int/
url: https://doi.org/10.1101/2020.05.01.20077743 http://medrxiv.org/cgi/content/short/2020.05.01.20077743v1?rss=1
has_full_text: FALSE
G_ID: a_non_competing_pair_of_human_neutralizing_antibodies_block_covid_19_virus_binding